B Background: lip prints are one of the identification methods in forensicodontology that utilize the groove patterns on the vermilion border of the lips, whichare unique and relatively permanent. the accuracy of lip print interpretation isinfluenced by the duplication media used, and there is currently no standardizedmethod for optimal lip print recording. objective: this study aimed to determinewhether there are differences in lip print interpretation between duplication mediausing polyvinyl siloxane and lipstick. methods: this analytical observational studyemployed a cross-sectional design. a total of 120 lip print samples were obtainedfrom 60 subjects, with each subject recorded using both polyvinyl siloxane andlipstick. in the polyvinyl siloxane method, impressions were taken directly from thelips and poured with type ii dental stone. in the lipstick method, lipstick was appliedto the lips and transferred onto mica plastic using a single-motion technique.interpretation was assessed based on the clarity of lip print patterns and analyzedusing the mann–whitney test. results: reliability testing showed that both mediademonstrated very high reliability (almost perfect agreement). the mann–whitneytest revealed a statistically significant difference between the two duplication media(p < 0.05). conclusion: there is a significant difference in lip print interpretationbetween polyvinyl siloxane and lipstick.